Monoclonal antibody (MAb) based immunodot for early detection of Aphanomyces invadans in fish

Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field

A monoclonal antibody-based flow-through immunoassay (FTA) was developed using a nitrocellulose membrane placed on the top of adsorbent pads enclosed in a plastic cassette with a test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.) invadans. The FTA limit of detection was 7 μg/mL for A. invadan...

Immunofluorescence of the epizootic ulcerative syndrome pathogen, Aphanomyces invadans, using a monoclonal antibody.

A monoclonal antibody (MAb), designated 3gJC9, was raised against a protein antigen of Aphanomyces invadans, the oomycete pathogen that causes epizootic ulcerative syndrome (EUS). The antigen was expressed on the surface of hyphae and secreted extracellularly. MAb 3gJC9 did not cross-react with other oomycete or fungal pathogens of fish, although it did react to the crayfish plague pathogen A. ...

Short communication:In vitro screening of Sonneratia alba extract against the oomycete fish pathogen, Aphanomyces invadans

Molecular assays for detecting Aphanomyces invadans in ulcerative mycotic fish lesions.

The pathogenic oomycete Aphanomyces invadans is the primary etiological agent in ulcerative mycosis, an ulcerative skin disease caused by a fungus-like agent of wild and cultured fish. We developed sensitive PCR and fluorescent peptide nucleic acid in situ hybridization (FISH) assays to detect A. invadans. Laboratory-challenged killifish (Fundulus heteroclitus) were first tested to optimize and...

Effect of Mikania Cordata on Non-specific Immune Response and Survival of Labeo Rohita against Aphanomyces Invadans

The study was conducted to evaluate the efficacy of Mikania cordata leaf powder on non-specific immune response and disease resistance of Labeo rohita fingerlings against the Aphanomyces invadans infection. M. cordata extract was incorporated in the diets (at 0.0%, 1%, 2% and 3%) of L. rohita fingerlings (19±0.95g). Blood and serum sampling was carried out on 0th day, 14th day, 28th day and 42t...